【文獻標題】Dehydroepiandrosterone alleviates intestinal inflammatory damage via GPR30-mediated Nrf2 activation and NLRP3 inflammasome inhibition in colitis mice

【作者】Ji Cao , Miaomiao Lu , Weiyuan Yan , et.al

【作者單位】南京農(nóng)業(yè)大學(xué)（Nanjing Agricultural University）

【文獻中引用產(chǎn)品】

人腫瘤壞死因子α(TNF-α)ELISA試劑盒

人白細胞介素1β(IL-1β)ELISA試劑盒

人雌二醇(E2)ELISA試劑盒

【關(guān)鍵詞】Dehydroepiandrosterone ,GPR30 ,Nrf2 ,NLRP3 inflammasome ,Colitis

【DOI】10.1016/j.freeradbiomed.2021.06.025

【影響因子(IF)】7.37

【出版期刊】《Free Radical Biology and Medicine》

【產(chǎn)品原文引用】

Sigma (St Louis, MO, USA) provided the DHEA, dimethyl sulfoxide (DMSO) and LPS (Escherichia coli 055:B5). The Opti-MEM medium and fetal bovine serum (FBS) were obtained from Gibco (Erie, NY, USA). The dulbecco's modified Eagle's medium (DMEM) and trypsin-EDTA were all obtained from Biological Industries (Kibbutz Beit-Haemek, Israel). Corning (NY, USA) provided the cell culture dishes.

TRIZOL Reagent Kits, SYBR Green Master Mix and reverse transcriptase kit were all purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Jiancheng Bioengineering Institute of Nanjing (Nanjing, China) provided the Nitric Oxide (NO), malondialdehyde (MDA) and total superoxide dismutase (SOD) detection kits. Shanghai hengyuan biological technology Co., Ltd. (Shanghai, China) provided the

TNF-α, IL-1β and Estradiol (E2) ELISA kits. Beyotime Biotechnology Institute (Shanghai, China) provided the glutathione peroxidase, nitric Oxide (NO), protein contents assay kits and N-Acetyl-L-cysteine (NAC). MCE (St. Louis, MO, USA) provided the inhibitors including G1, G15, SB203580, U0126, BAY11-7082, GKT137831, TAK-242, 3-MA, Fulvestrant, Flutamide, ML385 and RTA-408.

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